Each substrate forms a 10-base-pair (bp) duplex with nucleotides 2–11 of the 12-nucleotide guide counted from box D/D′. The guide–substrate duplexes are placed in an RNA-binding channel formed by the ...
Mutant alleles used were plt1-4 plt2-2 in the ecotype Wassilewskija (Ws) and scr-4 (Ws). Seedlings at four days post germination were excised by hand under a dissecting microscope using a 30G sterile ...